Difference between revisions of "Part:BBa K4214001"

 
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<partinfo>BBa_K4214001 short</partinfo>
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<partinfo>BBa_K4214000 short</partinfo>
  
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Gastric Intrinsic Factor with signal peptide and polyhistidine tag. The signal peptide is 22 AA long and allows for the secretion of the protein in the body.
 
  
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This part codes for human Gastric Intrinsic Factor. We used the human sequence of the gene. The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4214001 SequenceAndFeatures</partinfo>
 
  
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==Usage and Biology==
  
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<!-- Uncomment this to enable Functional Parameter display
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*NCBI page of the human gastric intrinsic factor [https://www.ncbi.nlm.nih.gov/gene/2694]
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===Functional Parameters===
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*We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia
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<partinfo>BBa_K4214001 parameters</partinfo>
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*We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli
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*This new part can be found here. (link for new part )

Revision as of 17:16, 8 October 2022

Human Gastric Intrinsic Factor (hGIF) 


This part codes for human Gastric Intrinsic Factor. We used the human sequence of the gene. The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.


Usage and Biology

  • NCBI page of the human gastric intrinsic factor [1]
  • We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia
  • We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli
  • This new part can be found here. (link for new part )