Difference between revisions of "Part:BBa K4368006:Design"
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===Source===
 
===Source===
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This part was derived from the reporter device used by Elowitz's repressilator Elowitz. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers Andersen to build the reporter device. Andersen constructed those GFP variants based on a gfpmut3b containing plasmid that was a gift from R. H. Valdivia Valdivia. The PCR method used in construction by Andersen changed aa residue 2 from serine to arginine with no detectable phenotypic effect in their experiments.
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The ''GFP'' gene is an optimized synthetic construction based on: <partinfo>BBa_E1010</partinfo>.
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===References===
 
===References===

Revision as of 18:19, 7 October 2022


pcstA + rbs + GFP + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 804


Design Notes

No special considerations.


Source

The GFP gene is an optimized synthetic construction based on: BBa_E1010.


References