Difference between revisions of "Part:BBa T2025"

Latest revision as of 18:22, 17 September 2009

Glutathione S-transferase internal domain

This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and detect of proteins.

Usage and Biology

The glutathione S-transferase (GST) protein domain enables one-step non-denaturing purification of proteins using a glutathione-agarose column. The GST protein domain can be places on either the N- or C-terminus of proteins. In some cases, fusion to GST can increase the expression and/or solubility of the fused protein. GST can also be used to immobilize proteins.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_T2025 short</partinfo>
-This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and/or measure the quantity of proteins.
+This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and detect of proteins.
 ===Usage and Biology===
-The glutathione S-transferase (GST) protein domain enables one-step non-denaturing purification of proteins using a glutathione-agarose column.  The GST protein domain can be places on either the N- or C-terminus of proteins.
+The glutathione S-transferase (GST) protein domain enables one-step non-denaturing purification of proteins using a glutathione-agarose column.  The GST protein domain can be places on either the N- or C-terminus of proteins.  In some cases, fusion to GST can increase the expression and/or solubility of the fused protein.  GST can also be used to immobilize proteins.
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