Difference between revisions of "Part:BBa T2028"

Revision as of 18:15, 17 September 2009

Chitin binding internal domain

This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins.

Usage and Biology

The chitin binding domain tag is used for protein purification using a chitin column. CBD can be placed on either the N- or C-terminus of proteins and is usually used in conjunction with a self-splicing intein tag. Purification of a CBD-tagged protein is a one-step purification process that yields very pure protein with low miligram yields. The primary disadvantage is that the purification must be done in the absence of thiol-containing reducing agents until the elution step. However, the matrix is compatible with high salt and nonionic detergents.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-The chitin binding domain tag is used for protein purification using a chitin column. CBD can be placed on either the N- or C-terminus of proteins. Purification of a CBD-tagged protein is a one-step purification process that yields very pure protein with low miligram yields. The primary disadvantage is that the purification must be done in the absence of thiol-containing reducing agents until the elution step.  However, the matrix is compatible with high salt and nonionic detergents.
+The chitin binding domain tag is used for protein purification using a chitin column. CBD can be placed on either the N- or C-terminus of proteins and is usually used in conjunction with a self-splicing intein tag. Purification of a CBD-tagged protein is a one-step purification process that yields very pure protein with low miligram yields. The primary disadvantage is that the purification must be done in the absence of thiol-containing reducing agents until the elution step.  However, the matrix is compatible with high salt and nonionic detergents.
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