Difference between revisions of "Part:BBa K4368003"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4368003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4368003 SequenceAndFeatures</partinfo> | ||
− | + | ==Contribution == | |
+ | *'''Group:''' [https://2022.igem.wiki/uma-malaga/index.html] | ||
+ | *'''Author:''' Molina Calvo, Alonso | ||
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Revision as of 09:29, 6 October 2022
pcstA + rbs + bglX + terminator
BglX encodes for the β-glucosidase gene of Escherichia coli (BBa_K4368002). This enzyme is responsible of the degradation of celullose working coordinated with th genes cenA and cex. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1609
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1457
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1367
Contribution
- Group: [1]
- Author: Molina Calvo, Alonso