Difference between revisions of "Part:BBa K4115045"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4115045 short</partinfo>
 
<partinfo>BBa_K4115045 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1
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|-
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|'''Function'''
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|Transporter membrane protein
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|-
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|'''Use in'''
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|E.coli
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10], RFC 10 compatible
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|-
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|'''Backbone'''
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|pUC57<br>
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|-
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|'''Submitted by'''
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|[https://2022.igem.wiki/shanghaitech-china/ ShanghaiTech_China]
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|}
  
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This composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. The core component of this composite part is cscB(BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.
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This composite part entered S.elongatus HL7942 by natural transformation with E. coli with the help of two homology arms NS3-1 [https://parts.igem.org/Part:BBa_K3228025 (BBa_K3228025)] and NS3-2 [https://parts.igem.org/Part:BBa_K3971009 (BBa_K3971009)], which originally come from UTEX2973. The core component of this composite part is cscB [https://parts.igem.org/Part:BBa_K3971011 (BBa_K3971011)], a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. It can be expressed by a constitutive promoter J23101[https://parts.igem.org/Part:BBa_J23101 (J23101)]. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.
  
  

Revision as of 12:10, 3 October 2022


NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1

NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1
Function Transporter membrane protein
Use in E.coli
RFC standard RFC 10, RFC 10 compatible
Backbone pUC57
Submitted by ShanghaiTech_China

This composite part entered S.elongatus HL7942 by natural transformation with E. coli with the help of two homology arms NS3-1 (BBa_K3228025) and NS3-2 (BBa_K3971009), which originally come from UTEX2973. The core component of this composite part is cscB (BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. It can be expressed by a constitutive promoter J23101(J23101). We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
    Illegal NgoMIV site found at 1678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1722