Difference between revisions of "Part:BBa K4115040"

Revision as of 11:36, 3 October 2022

J23101-B0034-LuxI-B0015

J23101-B0034-LuxI-B0015
Function	gene regulator
Use in	E.coli
RFC standard	RFC 10, RFC 10 compatible
Backbone	pBBR1
Submitted by	(ShanghaiTech_China)

This composite part is used to produce 3OC6 HSL by LacI (BBa_C0161) and send it to the receiver protein LuxR (BBa_C0062)in the other E.coli.When the LuxR receives the signal, it will regulate and express the downstream report gene sfGFP, thus we can detect whether LuxI is functional by observing the fluorescence intensity under UV light.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4115040 short</partinfo>
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
+! colspan="2" style="background:#FFBF00;"|J23101-B0034-LuxI-B0015
+|-
+|'''Function'''
+|gene regulator
+|-
+|'''Use in'''
+|E.coli
+|-
+|'''RFC standard'''
+|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10], RFC 10 compatible
+|-
+|'''Backbone'''
+|pBBR1<br>
+|-
+|'''Submitted by'''
+|[https://2022.igem.wiki/shanghaitech-china/ (ShanghaiTech_China)]
+|}
-none
+This composite part is used to produce 3OC6 HSL by LacI [https://parts.igem.org/Part:BBa_C0161 (BBa_C0161)] and send it to the receiver protein LuxR [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)]in the other E.coli.When the LuxR receives the signal, it will regulate and express the downstream report gene sfGFP, thus we can detect whether LuxI is functional by observing the fluorescence intensity under UV light.
 <!-- Add more about the biology of this part here