Difference between revisions of "Part:BBa T2017"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_T2017 short</partinfo> | <partinfo>BBa_T2017 short</partinfo> | ||
| Line 5: | Line 4: | ||
This is a S-tag special internal domain designed to between protein domains. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins. | This is a S-tag special internal domain designed to between protein domains. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The protease subtilisin cleaves a single peptide bond in native RNase A. The resulting two fragments are the S-peptide (residues 1-20 of RNase A) and the S-protein (residues 21-124 of RNase A). These two fragments noncovalently associate to form a functional ribonuclease, called RNase S. | ||
| + | |||
| + | The S-tag system was developed based on the high affinity between the S-peptide and S-protein of RNase S. The S-tag is derived from the first 15 residues of the S-peptide and therefore was originally called S15<cite>Kim93</cite>. It binds with high affinity to the S-protein (or S-fragment) of RNase A. Elution from the S-protein occurs at low pH. An RNase A assay is possible for a quantitative assay of protein expression levels. Colorimetric assays are also available for detection of S-tagged proteins without the use of an antibody. | ||
| + | |||
| + | The S-tag can be used at the N- or C-terminus or internal to a protein of interest. | ||
<!-- --> | <!-- --> | ||
Latest revision as of 13:10, 3 September 2009
S-tag special internal domain (KETAAAKFERQHMDS)
This is a S-tag special internal domain designed to between protein domains. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Usage and Biology
The protease subtilisin cleaves a single peptide bond in native RNase A. The resulting two fragments are the S-peptide (residues 1-20 of RNase A) and the S-protein (residues 21-124 of RNase A). These two fragments noncovalently associate to form a functional ribonuclease, called RNase S.
The S-tag system was developed based on the high affinity between the S-peptide and S-protein of RNase S. The S-tag is derived from the first 15 residues of the S-peptide and therefore was originally called S15Kim93. It binds with high affinity to the S-protein (or S-fragment) of RNase A. Elution from the S-protein occurs at low pH. An RNase A assay is possible for a quantitative assay of protein expression levels. Colorimetric assays are also available for detection of S-tagged proteins without the use of an antibody.
The S-tag can be used at the N- or C-terminus or internal to a protein of interest.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 10
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4