Difference between revisions of "Part:BBa T2015"
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− | The S-tag binds to the S-fragment of RNase A and is eluted at low pH. | + | The S-tag binds to the S-fragment of RNase A and is eluted at low pH. An RNase A assay is possible for a quantitative assay of protein expression levels. Colorimetric assays are also available for detection of S-tagged proteins without the use of an antibody. |
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Revision as of 20:46, 2 September 2009
S-tag tail domain (KETAAAKFERQHMDS)
This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
Usage and Biology
The S-tag binds to the S-fragment of RNase A and is eluted at low pH. An RNase A assay is possible for a quantitative assay of protein expression levels. Colorimetric assays are also available for detection of S-tagged proteins without the use of an antibody.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 10
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4