Difference between revisions of "Part:BBa K4226005"
Line 19: Line 19:
 
<partinfo>BBa_K4226005 parameters</partinfo>
 
<partinfo>BBa_K4226005 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
 +
<b>Reference</b>
 +
<br>
 +
[1] Bai, Jiuyuan, et al. "A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering." Nature communications 11.1 (2020): 1-14.
 +
[2] Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for cellular imaging." Nature methods 14.1 (2017): 53-56.<br>

Revision as of 05:54, 28 September 2022


RNA and protein reporting systems

3WJ-Bro and mScarlet-I together were used as RNA and protein reporting systems. 3WJ-Bro can be applied as a gene marker in creating a system for reporting the presence and expression of target genes. mScarlet-I is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 780
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference
[1] Bai, Jiuyuan, et al. "A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering." Nature communications 11.1 (2020): 1-14. [2] Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for cellular imaging." Nature methods 14.1 (2017): 53-56.