Difference between revisions of "Part:BBa K4143340"
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This composite part includes an antimicrobial peptide (HBCM2), TEV protease site, targeting peptide, and T4GALA encapsulin. The encapsulin is a protein nanocompartment that sequesters cargo carrying a targeting peptide. In this case, the antimicrobial peptide (AMP) is the cargo and it's attached to the targeting peptide to be sequestered within the encapsulin. This serves to protect the bacterial host from the AMP that it's producing. The TEV protease site attaches the targeting peptide and AMP so the AMP can be isolated later on in downstream purification.   
 
This composite part includes an antimicrobial peptide (HBCM2), TEV protease site, targeting peptide, and T4GALA encapsulin. The encapsulin is a protein nanocompartment that sequesters cargo carrying a targeting peptide. In this case, the antimicrobial peptide (AMP) is the cargo and it's attached to the targeting peptide to be sequestered within the encapsulin. This serves to protect the bacterial host from the AMP that it's producing. The TEV protease site attaches the targeting peptide and AMP so the AMP can be isolated later on in downstream purification.   
  
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===Usage and Biology===
 
  
 
[[File:Experiment-construct.png|400px]]
 
[[File:Experiment-construct.png|400px]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4143340 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4143340 SequenceAndFeatures</partinfo>

Revision as of 20:51, 27 September 2022


AMP Encapsulin Composite

This composite part includes an antimicrobial peptide (HBCM2), TEV protease site, targeting peptide, and T4GALA encapsulin. The encapsulin is a protein nanocompartment that sequesters cargo carrying a targeting peptide. In this case, the antimicrobial peptide (AMP) is the cargo and it's attached to the targeting peptide to be sequestered within the encapsulin. This serves to protect the bacterial host from the AMP that it's producing. The TEV protease site attaches the targeting peptide and AMP so the AMP can be isolated later on in downstream purification.


Experiment-construct.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 191
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]