Difference between revisions of "Part:BBa K4260111"

(→‎Design)
(→‎Design)
Line 14: Line 14:
 
<html> <p align="justify">
 
<html> <p align="justify">
  
−
This RecA intein ESR1 biosensor consists of two inteins capable of joining and separating from two protein fragments. Considering this, the ESR1 biosensor gene coding for the hERalpha protein was introduced with its respective linker, in such a way that when an endocrine disruptor binds to the biosensor, the inteins join and separate with the rest of the protein, removing the linker, the biosensor and the two inteins. The two endogenous fragments encode the chromoprotein AmilCP <html><a href='https://parts.igem.org/Part:BBa_K592009'>BBa_K592009</a></html>)].
+
This RecA intein ESR1 biosensor consists of two inteins capable of joining and separating from two protein fragments. Considering this, the ESR1 biosensor gene coding for the hERalpha protein was introduced with its respective linker, in such a way that when an endocrine disruptor binds to the biosensor, the inteins join and separate with the rest of the protein, removing the linker, the biosensor and the two inteins. The two endogenous fragments encode the chromoprotein AmilCP [<html><a href='https://parts.igem.org/Part:BBa_K592009'>BBa_K592009</a></html>].
 
<p align="justify">
 
<p align="justify">
  

Revision as of 17:36, 27 September 2022


RecA mini intein mediated ESR1 protein with OmpA signal peptide, linker and AmilCP.

This part encodes an intein mediated biosensor, consisting of a divided AmilCP chromoprotein gene, a mutated intein RecA N-terminal and C-terminal domains and a ESR1 Ligand Binding Domain. The RecA intein comes from Mycobacterium Tuberculosis (Mtu) which has been mutated with the purpose of making it more stable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Fig.x:RecA intein behavior when an EDC is present.

This RecA intein ESR1 biosensor consists of two inteins capable of joining and separating from two protein fragments. Considering this, the ESR1 biosensor gene coding for the hERalpha protein was introduced with its respective linker, in such a way that when an endocrine disruptor binds to the biosensor, the inteins join and separate with the rest of the protein, removing the linker, the biosensor and the two inteins. The two endogenous fragments encode the chromoprotein AmilCP [BBa_K592009].