Difference between revisions of "Part:BBa K4226004"
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*We applied a typical cellular sensor that can be abstracted as a three-stage processor comprising a sensing module that recognizes and transduces external signals into intracellular transcriptional signals, a computing module that modulates the transduced sensor signals, and an output actuating module that executes physiological responses. | *We applied a typical cellular sensor that can be abstracted as a three-stage processor comprising a sensing module that recognizes and transduces external signals into intracellular transcriptional signals, a computing module that modulates the transduced sensor signals, and an output actuating module that executes physiological responses. | ||
*We first used the promoter of sulA as sensor module. The amplifier module (Amp30E Amplification Device) includes hrpR, hrpS and PhrpL , and the eGFP was used as an output module to test the effect of amplifier module. | *We first used the promoter of sulA as sensor module. The amplifier module (Amp30E Amplification Device) includes hrpR, hrpS and PhrpL , and the eGFP was used as an output module to test the effect of amplifier module. | ||
| + | *The gene circuits is shown bellow: | ||
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| + | [[File:PSB1C3-sulAp-Amp30E-eGFP.png|600px|thumb|center|Gene circuit: PSB1C3-sulAp-Amp30E-eGFP.png]]<br> | ||
Revision as of 05:31, 27 September 2022
Amp30E Amplification Device
The Amp30E Amplification Device is an amplifier module includes hrpR Gene, hrpS Gene and hrpL Promoter, this engineered multi-layered transcriptional amplifiers can be able to sequentially boost output expression level of target genes.
Usage and Biology
Characterized by CAFA_China 2022
- We applied a typical cellular sensor that can be abstracted as a three-stage processor comprising a sensing module that recognizes and transduces external signals into intracellular transcriptional signals, a computing module that modulates the transduced sensor signals, and an output actuating module that executes physiological responses.
- We first used the promoter of sulA as sensor module. The amplifier module (Amp30E Amplification Device) includes hrpR, hrpS and PhrpL , and the eGFP was used as an output module to test the effect of amplifier module.
- The gene circuits is shown bellow:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1891
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal SapI.rc site found at 1734
Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.