Difference between revisions of "Part:BBa K4432130:Design"

 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4432136 short</partinfo>
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<partinfo>BBa_K4432130 short</partinfo>
  
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<partinfo>BBa_K4432136 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4432130 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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to do
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T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) + toehold switches stem loop RBS ([[Part:BBa_K3453005|BBa_K3453005]]) + CymA coding sequence ([[Part:BBa_K4432036|BBa_K4432036]]) + the strong SBa_000587 Terminator ([[Part:BBa_K3453000|BBa_K3453000]])
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===Source===
 
===Source===
  
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to do
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DNA synthesis & Assembly
  
 
===References===
 
===References===

Latest revision as of 21:58, 26 September 2022


CymA expression cassette under the control of the T7 promoter with the synthetic RBS stem-loop desig


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter (BBa_K2150031) + toehold switches stem loop RBS (BBa_K3453005) + CymA coding sequence (BBa_K4432036) + the strong SBa_000587 Terminator (BBa_K3453000)

Source

DNA synthesis & Assembly

References