Difference between revisions of "Part:BBa K4432321:Design"

 
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===Design Notes===
 
===Design Notes===
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to do
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T5 promoter ([[Part:BBa_K4432000|BBa_K4432000]]) + custom made RBS ([[Part:BBa_K4432011|BBa_K4432011]]) + CrtO coding sequence ([[Part:BBa_K4432021|BBa_K4432021]]) + custom made RBS ([[Part:BBa_K4432012|BBa_K4432012]]) + idi coding sequence ([[Part:BBa_K3166068|BBa_K3166068]]) + custom made RBS ([[Part:BBa_K4432013|BBa_K4432013]]) + dxs coding sequence ([[Part:BBa_K3166061|BBa_K3166061]])
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+
−
 
+
  
 
===Source===
 
===Source===
  
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to do
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DNA synthesis & Assembly
  
 
===References===
 
===References===

Revision as of 21:13, 26 September 2022


CrtO, idi and dsx expression operon under the control of the T5 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2288
    Illegal BamHI site found at 2295
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2114
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2114
    Illegal NgoMIV site found at 569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3049


Design Notes

T5 promoter (BBa_K4432000) + custom made RBS (BBa_K4432011) + CrtO coding sequence (BBa_K4432021) + custom made RBS (BBa_K4432012) + idi coding sequence (BBa_K3166068) + custom made RBS (BBa_K4432013) + dxs coding sequence (BBa_K3166061)

Source

DNA synthesis & Assembly

References