Coding

Part:BBa_K4432021

Designed by: Paul Weimer   Group: iGEM22_Evry_Paris-Saclay   (2022-09-26)


CrtO: β−carotene ketolase

This part is an Escherichia coli codon optimized version of the ß-carotene-ketolase (CrtO-type) from Dietzia psychralcaliphila ILA-1 (GenBank: AWH95158.1).

Usage and Biology

The ß-carotene-ketolase (EC 1.14.99.63) catalyses the conversion of ß-carotene into echinenone and of echinenone into canthaxanthin (Figure 1).

Figure 1. Conversion of ß-carotene into canthaxanthin by EC: 1.14.99.63.

The Dietzia psychralcaliphila CrtO is a 55.631 kDa protein of 528 amino acids. It's function was not yet described in the literature.

When placed under the control of the hybrid T5 promoter regulated by LacI (BBa_K4432000) or the classical pLac promoter (BBa_R0010), this part leads to the synthesis of canthaxanthin in an E. coli producing ß-carotene.

For further details, see the Main Pages on the Registry of the CrtW expression plasmids (BBa_K4432121, BBa_K4432221 and BBa_K4432321).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 454
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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