Difference between revisions of "Part:BBa K4202004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | <div>Replacing codons in the original mRNA sequence with codons that are used frequently in <i>Bacillus subtilis</i> to ensure that the codons in the newly designed mRNA sequence are more compatible with the codon usage bias of <i>Bacillus subtilis</i>, avoiding the emergence of rare codons. | + | <div>Replacing codons in the original mRNA sequence with codons that are used frequently in <i>Bacillus subtilis</i> to ensure that the codons in the newly designed mRNA sequence are more compatible with the codon usage bias of <i>Bacillus subtilis</i>, avoiding the emergence of rare codons. |
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===Source=== | ===Source=== | ||
| − | This part is created by codon optimization for <i>Bacillus subtilis</i> from Part:<a href="https://parts.igem.org/Part:BBa_K2232000:Design" target="_blank" rel="noopener noreferrer">BBa_K2232000</a>. | + | <div>This part is created by codon optimization for <i>Bacillus subtilis</i> from Part:<a href="https://parts.igem.org/Part:BBa_K2232000:Design" target="_blank" rel="noopener noreferrer">BBa_K2232000</a>.</div> |
===References=== | ===References=== | ||
Faridi, S., & Satyanarayana, T. (2016). Characteristics of recombinant α-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1. <i>International journal of biological macromolecules</i>, 89, 659-668. | Faridi, S., & Satyanarayana, T. (2016). Characteristics of recombinant α-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1. <i>International journal of biological macromolecules</i>, 89, 659-668. | ||
Revision as of 11:56, 25 September 2022
TSLV-BS-CA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 540
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 166
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 535
Design Notes
Replacing codons in the original mRNA sequence with codons that are used frequently in Bacillus subtilis to ensure that the codons in the newly designed mRNA sequence are more compatible with the codon usage bias of Bacillus subtilis, avoiding the emergence of rare codons.
Source
This part is created by codon optimization for Bacillus subtilis from Part:<a href="https://parts.igem.org/Part:BBa_K2232000:Design" target="_blank" rel="noopener noreferrer">BBa_K2232000</a>.