Difference between revisions of "Part:BBa J428100"

Revision as of 20:48, 8 June 2022

Interlab_NegC

Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.

Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.

It can be used as negative control for fluorescent measurements in general.

Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 71
Illegal NheI site found at 94
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_J428100 short</partinfo>
 Non-fluorescent negative control for InterLab studies 2022.
 It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR).
@@ Line 7: / Line 8: @@
 It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.
+Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.
-Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors
+It can be used as negative control for fluorescent measurements in general.
 [[File:EngCom_2022_J428100.png|500px|thumb|center|alt=J428100.|Figure 1. SBOL Visual representation of the device [https://parts.igem.org/Part:BBa_J428100 J428100] from [https://technology.igem.org/interlabs 2022 Interlab studies.] ]]