Difference between revisions of "Part:BBa K3977006"

 
 
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<partinfo>BBa_K3977006 parameters</partinfo>
 
<partinfo>BBa_K3977006 parameters</partinfo>
 
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==Contribution ==
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*'''Group:''' [https://2021.igem.org/Team:SCU-China SCU-China iGEM team 2021]
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*'''Author:''' Yilong Xu
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*'''Summary:''' Improve this part by expanding itself
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*'''Link to our biobrick: '' https://parts.igem.org/Part:BBa_K3977006
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===Characterization/Improvement ===
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Based on BBa_K3380153, we construct a RNA adaptor with 4 repeats of BBa_K3380153,
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We put them under J23100, and detect their performance in 488/527.
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Our result shows that BBa_K397706 show more output (C) , meaning that it performed better than original part BBa_K3380153 and our improvement is successful.
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[[image: T--SCU-China--scu-2021-4X.png|600px|center|]]
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                        Figure 1: Relative fluorescence intensity BBa_K3380153 and BBa_K3977006

Latest revision as of 21:39, 21 October 2021


3WJ 4XBRO

3WJ 4XBRO

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 199
    Illegal SpeI site found at 174
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 199
    Illegal SpeI site found at 174
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 199
    Illegal BamHI site found at 168
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 199
    Illegal SpeI site found at 174
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 199
    Illegal SpeI site found at 174
  • 1000
    COMPATIBLE WITH RFC[1000]


Contribution

Characterization/Improvement

Based on BBa_K3380153, we construct a RNA adaptor with 4 repeats of BBa_K3380153, We put them under J23100, and detect their performance in 488/527.

Our result shows that BBa_K397706 show more output (C) , meaning that it performed better than original part BBa_K3380153 and our improvement is successful.

T--SCU-China--scu-2021-4X.png
                        Figure 1: Relative fluorescence intensity BBa_K3380153 and BBa_K3977006