Difference between revisions of "Part:BBa K3861017"
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<img src="https://2021.igem.org/wiki/images/8/8c/T--Humboldt_Berlin--plldR_characterisation.png" alt="PlldR-GFP" width="500"> | <img src="https://2021.igem.org/wiki/images/8/8c/T--Humboldt_Berlin--plldR_characterisation.png" alt="PlldR-GFP" width="500"> |
Revision as of 19:03, 21 October 2021
PlldR-GFP
Introduction
Composite part containing the PlldR promoter (BBa_K1847008) fused to GFP (BBa_E0840) as a reporter gene. Both parts were already characterized in depth by other teams. We used this part in Salmonella Typhimurium.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 816
Characterization
We chara