Difference between revisions of "Part:BBa P1003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
*Used for [[Help:Plasmids/Construction|modular BioBrick vector construction]] using BioBrick base vector [[Part:BBa_I51020|BBa_I51020]]. | *Used for [[Help:Plasmids/Construction|modular BioBrick vector construction]] using BioBrick base vector [[Part:BBa_I51020|BBa_I51020]]. | ||
+ | |||
+ | ===MiamiU_OH Contribution=== | ||
+ | This part was optimized for expression and function in the photosynthetic chassis Synechococcus elongatus PCC 7942. The promoter can be found at <partinfo>BBa_K4047020</partinfo> and the coding sequence can be found at <partinfo>BBa_K4047019</partinfo>. | ||
===References=== | ===References=== |
Latest revision as of 20:42, 20 October 2021
kanamycin resistance cassette
Kanamycin resistance cassette including promoter and coding sequence. It lacks a terminator.
Usage and Biology
- Used for modular BioBrick vector construction using BioBrick base vector BBa_I51020.
MiamiU_OH Contribution
This part was optimized for expression and function in the photosynthetic chassis Synechococcus elongatus PCC 7942. The promoter can be found at BBa_K4047020 and the coding sequence can be found at BBa_K4047019.
References
Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656101 GenBank EU496093]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]