Difference between revisions of "Part:BBa K3740041"
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<p>This is a combination of SE (<partinfo>BBa_K3740042</partinfo>) and IMM (<partinfo>BBa_K3740034</partinfo>) into device, that is used to regulate the expression of SE and IMM.</p> | <p>This is a combination of SE (<partinfo>BBa_K3740042</partinfo>) and IMM (<partinfo>BBa_K3740034</partinfo>) into device, that is used to regulate the expression of SE and IMM.</p> | ||
<h3>Usage</h3> | <h3>Usage</h3> | ||
| − | <p>We connected PA1/04/03-RBS400-SE-B1006（<partinfo>BBa_K3740056</partinfo>）and J23118-RBSII-IMM-rrnB T1 (<partinfo>BBa_K3740053</partinfo>) to the expression vector pSEVA331 by standard assembly, and introduced the connection mixture into <i>G. | + | <p>We connected PA1/04/03-RBS400-SE-B1006（<partinfo>BBa_K3740056</partinfo>）and J23118-RBSII-IMM-rrnB T1 (<partinfo>BBa_K3740053</partinfo>) to the expression vector pSEVA331 by standard assembly, and introduced the connection mixture into <i>G. hansenii</i> ATCC 53582. when the engineering bacteria were lysed, the SE protein can be released to kill <i>Pseudomonas aeruginosa</i> specifically.</p> |
<h3>Characterization</h3> | <h3>Characterization</h3> | ||
<h4>1. Verification of SE protein antibacterial performance of composite parts</h4> | <h4>1. Verification of SE protein antibacterial performance of composite parts</h4> | ||
<p>It can be seen from Figure 1(a) that PA1/04/03-RBS300-SE-B1006-J23118-RBSII-IMM-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582 has no inhibitory effect on PAO1, and the zone of inhibition in (b) is not obvious.</p> | <p>It can be seen from Figure 1(a) that PA1/04/03-RBS300-SE-B1006-J23118-RBSII-IMM-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582 has no inhibitory effect on PAO1, and the zone of inhibition in (b) is not obvious.</p> | ||
[[File:szpt49.png|500px|thumb|center|Figure 1,（a）Supernatants from different bacterial strains lysate inhibited the growth of PAO1. (b) Inhibition zone experiment. (c) Strains are used for Figure(a) and Figure(b)]] | [[File:szpt49.png|500px|thumb|center|Figure 1,（a）Supernatants from different bacterial strains lysate inhibited the growth of PAO1. (b) Inhibition zone experiment. (c) Strains are used for Figure(a) and Figure(b)]] | ||
Latest revision as of 17:26, 20 October 2021
PA1/04/03-RBS400-SE-B1006-J23118-RBSII-IMM-rrnB T1
Description
This is a combination of SE and IMM into device, that is used to regulate the expression of SE and IMM.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2524
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2524
Illegal NheI site found at 2612
Illegal NheI site found at 2635 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2524
Illegal BglII site found at 2229
Illegal BamHI site found at 1801 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2524
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2524
Illegal NgoMIV site found at 1600
Illegal AgeI site found at 1690 - 1000COMPATIBLE WITH RFC[1000]
2021 SZPT-China
Biology
This is a combination of SE (BBa_K3740042) and IMM (BBa_K3740034) into device, that is used to regulate the expression of SE and IMM.
Usage
We connected PA1/04/03-RBS400-SE-B1006（BBa_K3740056）and J23118-RBSII-IMM-rrnB T1 (BBa_K3740053) to the expression vector pSEVA331 by standard assembly, and introduced the connection mixture into G. hansenii ATCC 53582. when the engineering bacteria were lysed, the SE protein can be released to kill Pseudomonas aeruginosa specifically.
Characterization
1. Verification of SE protein antibacterial performance of composite parts
It can be seen from Figure 1(a) that PA1/04/03-RBS300-SE-B1006-J23118-RBSII-IMM-rrnB T1-pSEVA331-G. hansenii ATCC 53582 has no inhibitory effect on PAO1, and the zone of inhibition in (b) is not obvious.
