Difference between revisions of "Part:BBa K3889102"

Latest revision as of 14:13, 19 October 2021

Ovastacin expression cassette Bacillus subtilis

P43 (BBa_K143013) fused with P22 binding site L (BBa_K3889091) and RBS (BBa_K1351030) in order to create P22 c2 repressed Ovastacin expression. Also in order to secrete Ovastacin PhoD signal peptide (BBa_K3889050) was also fused.

Usage and Biology

This part along with BBa_K3889100 and BBa_K3889101 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3889102 short</partinfo>
-P43 (BBa_K143013) fused with P22 binding site L (BBa_K3889091) and RBS (BBa_K1351030) in order to create P22 c2 repressed Ovastaci expression. Also in order to secrete Ovastacin PhoD signal peptide (<partinfo>BBa_K3889050</partinfo>) was also fused.
+P43 (<partinfo>BBa_K143013</partinfo>) fused with P22 binding site L (<partinfo>BBa_K3889091</partinfo>) and RBS (<partinfo>BBa_K1351030</partinfo>) in order to create P22 c2 repressed Ovastacin expression. Also in order to secrete Ovastacin PhoD signal peptide (<partinfo>BBa_K3889050</partinfo>) was also fused.
 ===Usage and Biology===