Difference between revisions of "Part:BBa K3889100"
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SRTF1 is expressed under a constitutive promoter SP126 (<partinfo>BBa_K3889010</partinfo>).  
 
SRTF1 is expressed under a constitutive promoter SP126 (<partinfo>BBa_K3889010</partinfo>).  
 
In absence of progesterone it can bind to its binding site (<partinfo>BBa_K3889030</partinfo>) and repress transcription of the gene.Now as progesterone enters the system SRTF1 binds to progesterone leaving its binding site free for the transcription machinery to start working. This results in a progesterone inducible gene expression.
 
In absence of progesterone it can bind to its binding site (<partinfo>BBa_K3889030</partinfo>) and repress transcription of the gene.Now as progesterone enters the system SRTF1 binds to progesterone leaving its binding site free for the transcription machinery to start working. This results in a progesterone inducible gene expression.
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===Usage and Biology===
  
 
This part along with <partinfo>BBa_K3889101</partinfo> and <partinfo>BBa_K3889102</partinfo> was used in order to produce Ovastacin (<partinfo>BBa_K3889022</partinfo>) when progesterone in absent in the fallopian tube. The working of the system is shown below:
 
This part along with <partinfo>BBa_K3889101</partinfo> and <partinfo>BBa_K3889102</partinfo> was used in order to produce Ovastacin (<partinfo>BBa_K3889022</partinfo>) when progesterone in absent in the fallopian tube. The working of the system is shown below:
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</video>
 
</video>
 
</html>
 
</html>
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889100 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889100 SequenceAndFeatures</partinfo>

Revision as of 11:12, 19 October 2021


SRTF1 expression cassette Bacillus subtilis

SRTF1 is expressed under a constitutive promoter SP126 (BBa_K3889010). In absence of progesterone it can bind to its binding site (BBa_K3889030) and repress transcription of the gene.Now as progesterone enters the system SRTF1 binds to progesterone leaving its binding site free for the transcription machinery to start working. This results in a progesterone inducible gene expression.

Usage and Biology

This part along with BBa_K3889101 and BBa_K3889102 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]