Difference between revisions of "Part:BBa K3740035"

 
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<partinfo>BBa_K3740035 short</partinfo>
 
<partinfo>BBa_K3740035 short</partinfo>
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===Description===
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This composite part is a generator consisting of pDawn and RFP. pDawn can induce expression in response to blue light.
  
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Co-expression with toxin protein protects the host
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===Sequence and Features===
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K3740035 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3740035 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K3740035 parameters</partinfo>
 
<partinfo>BBa_K3740035 parameters</partinfo>
 
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=2021 SZPT-China=
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<h3>Biology</h3>
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<p>This composite part is a generator consisting of pDawn (<partinfo>BBa_K1075044</partinfo>) and RFP(<partinfo>BBa_E1010</partinfo>). pDawn can induce expression in response to blue light.</p>
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<h3>Usage</h3>
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<p>Analyze the system by inserting the red fluorescent reporter protein RFP (<partinfo>BBa_E1010</partinfo>), assemble it on the pSEVA331 expression vector, and transfer the connection mixture to <i>G. haensenii</i> ATCC 53582. In this way, we can verify the blue light response function of the pDawn system in <i>G. hansenii</i> ATCC 53582.</p>
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[[File:szpt35.png|600px|thumb|center|Figure 1. Gene circuit of pDawn-B0034-RFP-rrnB T1]]
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<h3>Characterization</h3>
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<p>Red fluorescence could be observed in the pDawn-B0034-RFP-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582 under 470-nm blue light, but there was no red fluorescence under dark condition, <b>suggesting that the pDawn promoter could respond to blue light and induce gene expression in <i>G. hansenii</i> ATCC 53582.</b></p>
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[[File:szpt36.png|600px|thumb|center|Figure 2. Red fluorescence photo of the pDawn-B0034-RFP-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582 cultured under 470-nm blue light (a) or dark condition (b) for 24 hours.]]
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<h3>References</h3>
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<p>[1] Robert, Ohlendorf, Roee. Corrigendum to "From Dusk Till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression" [J. Mol. Biol. 416 (2012) 534–542] [J]. Journal of Molecular Biology, 2014.</p>
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Promoter(const.)-RBS34-YF1-FixJ-FixK-LambdaC-pC (pDawn)：<partinfo>BBa_K1075044</partinfo>

Revision as of 08:23, 19 October 2021


pDawn-B0034-RFP-rrnB T1

Description

This composite part is a generator consisting of pDawn and RFP. pDawn can induce expression in response to blue light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
    Illegal AgeI site found at 3997
    Illegal AgeI site found at 4109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI.rc site found at 525


2021 SZPT-China

Biology

This composite part is a generator consisting of pDawn (BBa_K1075044) and RFP(BBa_E1010). pDawn can induce expression in response to blue light.

Usage

Analyze the system by inserting the red fluorescent reporter protein RFP (BBa_E1010), assemble it on the pSEVA331 expression vector, and transfer the connection mixture to G. haensenii ATCC 53582. In this way, we can verify the blue light response function of the pDawn system in G. hansenii ATCC 53582.

Figure 1. Gene circuit of pDawn-B0034-RFP-rrnB T1

Characterization

Red fluorescence could be observed in the pDawn-B0034-RFP-rrnB T1-pSEVA331-G. hansenii ATCC 53582 under 470-nm blue light, but there was no red fluorescence under dark condition, suggesting that the pDawn promoter could respond to blue light and induce gene expression in G. hansenii ATCC 53582.

Figure 2. Red fluorescence photo of the pDawn-B0034-RFP-rrnB T1-pSEVA331-G. hansenii ATCC 53582 cultured under 470-nm blue light (a) or dark condition (b) for 24 hours.

References

[1] Robert, Ohlendorf, Roee. Corrigendum to "From Dusk Till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression" [J. Mol. Biol. 416 (2012) 534–542] [J]. Journal of Molecular Biology, 2014.

Promoter(const.)-RBS34-YF1-FixJ-FixK-LambdaC-pC (pDawn)：BBa_K1075044