Difference between revisions of "Part:BBa K3893029"
 
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[[File:T--Ecuador--K3893029_sbolv.png|500px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device ]]
 
[[File:T--Ecuador--K3893029_sbolv.png|500px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device ]]
  
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This device was used to characterize the pLux promoter. It is an AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
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This device is an AHL induced transcriptional unit to express phiX174 lysis protein together with a TU for the constitutive expression of LuxI. It was assembled with a one-pot Level 2 Golden Gate reaction using BsmBI type IIS endonuclease.
  
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This device is composed of the following standardized composite parts:  
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This composite part, together with [https://parts.igem.org/Part:BBa_K3893028 K3893028], is an intermediate composite part to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030 K3893030]) of our Agrobactory 593. It includes a TU that expresses GFP under rhe control of the pLux promoter and a TU that expresses LuxR constitutively. It is the first half of our population oscillator release device.
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This transcriptional unit is composed of the following standardized composite parts:  
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===Usage and Biology===
 
===Usage and Biology===
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This composite part, together with [https://parts.igem.org/Part:BBa_K3893028 K3893028], is an intermediate composite part to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030 K3893030]) of our Agrobactory 593. It includes a TU that expresses phiX174 lysis protein under the control of the pLux promoter and a TU that expresses LuxI constitutively. It is the second half of our population oscillator release device.
  
 
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Latest revision as of 19:17, 16 October 2021


AHL induced expression of phiX174 lysis protein TU and luxI constitutive expression TU

domestication.
Figure 1. SBOL Visual representation of the device

This device is an AHL induced transcriptional unit to express phiX174 lysis protein together with a TU for the constitutive expression of LuxI. It was assembled with a one-pot Level 2 Golden Gate reaction using BsmBI type IIS endonuclease.

This device is composed of the following standardized composite parts:

  • BBa_K3893026: a Level 1 transcriptional unit constitutively expressing luxI gene (Golden Braid compatible)
  • BBa_K3893027: a Level 1 transcriptional unit expresing phiX174 lysis protein under the control of the pux promoter (Golden Braid compatible)


Usage and Biology

This composite part, together with K3893028, is an intermediate composite part to assemble the release system device (K3893030) of our Agrobactory 593. It includes a TU that expresses phiX174 lysis protein under the control of the pLux promoter and a TU that expresses LuxI constitutively. It is the second half of our population oscillator release device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]