Difference between revisions of "Part:BBa K3893028"

 
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[[File:T--Ecuador--K3893028_sbolv.png|400px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device ]]
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[[File:T--Ecuador--K3893087_sbolv.png|400px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device ]]
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This devise was used to characterize the pLux promoter  
 
This devise was used to characterize the pLux promoter  
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
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<partinfo>BBa_K3893028 parameters</partinfo>
 
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===Usage and Biology===
 
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Revision as of 14:46, 16 October 2021

AHL induced expression of GFP (including luxR constitutive expression)

domestication.
Figure 1. SBOL Visual representation of the device

This devise was used to characterize the pLux promoter AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.


This composite part, together with K3893027, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized composite parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656122: a Level 1 transcriptional unit expresing GFP under the control of the pux promoter (Golden Braid compatible)
  • BBa_K2656114: a Level 1 transcriptional unit constitutively expressing luxR gene (Golden Braid compatible)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 956
    Illegal NheI site found at 979
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]