Difference between revisions of "Part:BBa K4047005"
Anniequyan (Talk | contribs) |
|||
Line 18: | Line 18: | ||
<!-- --> | <!-- --> | ||
===Long Description=== | ===Long Description=== | ||
− | This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase. The overlap sequence | + | This is the forward primer pairing with the reverse primer BBa_K4047004, to amplify the gene for fructose-1,6-bisphosphatase. This allows the insertion of both fructose-1,6-bisphosphatase and transaldolase into the pGEM-tal+fbp plasmid (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification. |
+ | |||
+ | This primer is entirely BioBrick compatible, making it readily useable for future iGEM teams. |
Latest revision as of 04:14, 16 October 2021
primerTF_fbp-foward
Forward primer for FBPase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Long Description
This is the forward primer pairing with the reverse primer BBa_K4047004, to amplify the gene for fructose-1,6-bisphosphatase. This allows the insertion of both fructose-1,6-bisphosphatase and transaldolase into the pGEM-tal+fbp plasmid (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.
This primer is entirely BioBrick compatible, making it readily useable for future iGEM teams.