Difference between revisions of "Part:BBa K4047002"
Line 17: Line 17:
 
<!-- -->
 
<!-- -->
 
===Long Description===
 
===Long Description===
−
This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
+
This is the forward primer paired with the reverse primer BBa_K4047003 to amplify the gene for fructose-1,6-bisphosphatase class 1 (found at Synpcc7942_2335 in GenBank for Synechococcus elongatus PCC 7942). The overlap sequence allows proper Gibson assembly with the pGEM-fbp backbone (BBa_K4047024) to create the full plasmid pGEM-fbp described in BBa_K4047037. The annealing portion (all caps) allows annealing to the wildtype S. elongatus PCC 7942 genome for proper amplification.

Revision as of 02:43, 16 October 2021


primerF_fbp-foward


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the forward primer paired with the reverse primer BBa_K4047003 to amplify the gene for fructose-1,6-bisphosphatase class 1 (found at Synpcc7942_2335 in GenBank for Synechococcus elongatus PCC 7942). The overlap sequence allows proper Gibson assembly with the pGEM-fbp backbone (BBa_K4047024) to create the full plasmid pGEM-fbp described in BBa_K4047037. The annealing portion (all caps) allows annealing to the wildtype S. elongatus PCC 7942 genome for proper amplification.