Difference between revisions of "Part:BBa K3733005"
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<partinfo>BBa_K3733005 short</partinfo>
 
<partinfo>BBa_K3733005 short</partinfo>
  
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<p>J23106a is a constitutive promoter obtained by mutating <a url="https://parts.igem.org/Part:BBa_J23106"> J23106</a>. The strength of this promoter is much weaker than J23106. </p>
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<p>J23106a is a constitutive promoter obtained by mutating <a href="https://parts.igem.org/Part:BBa_J23106"> J23106</a>. The strength of this promoter is much weaker than J23106. </p>
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<p>To characterize this part, J23106a and J23106 were cloned into pSC101 vector separately. We chose <a url="https://parts.igem.org/Part:BBa_K3733012">neGFP </a> as the reporter. Plasmids were transferred into <i>E.coli</i> DH5α. The E. coli strain was cultured overnight, and the relative intensity of J23106a was evaluated by measuring RFU/OD<sub>600</sub> under the control of two different promoters. The relative fluorescence unit (RFU) was determined by Synergy H1 microplate reader. </p>
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<p>To characterize this part, J23106a and J23106 were cloned into pSC101 vector separately. We chose <a href="https://parts.igem.org/Part:BBa_K3733012">neGFP </a> as the reporter. Plasmids were transferred into <i>E.coli</i> DH5α. The E. coli strain was cultured overnight, and the relative intensity of J23106a was evaluated by measuring RFU/OD<sub>600</sub> under the control of two different promoters. The relative fluorescence unit (RFU) was determined by Synergy H1 microplate reader. </p>
 
<div><img></img></div>
 
<div><img></img></div>
 
<p><b>Figure 2. </b> Comparison of relative strength between J23106 and J23106a.The microplate reader gain adopts 50</p>
 
<p><b>Figure 2. </b> Comparison of relative strength between J23106 and J23106a.The microplate reader gain adopts 50</p>

Revision as of 00:50, 15 October 2021


J23106a:Improved promoter of J23106

J23106a is a constitutive promoter obtained by mutating <a href="https://parts.igem.org/Part:BBa_J23106"> J23106</a>. The strength of this promoter is much weaker than J23106.


Functional Parameters

To characterize this part, J23106a and J23106 were cloned into pSC101 vector separately. We chose <a href="https://parts.igem.org/Part:BBa_K3733012">neGFP </a> as the reporter. Plasmids were transferred into E.coli DH5α. The E. coli strain was cultured overnight, and the relative intensity of J23106a was evaluated by measuring RFU/OD600 under the control of two different promoters. The relative fluorescence unit (RFU) was determined by Synergy H1 microplate reader. 

<img></img>

Figure 2. Comparison of relative strength between J23106 and J23106a.The microplate reader gain adopts 50

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]