Difference between revisions of "Part:BBa E0050"
Line 3: Line 3:
  
 
This part is a fusion protein between GFP (BBa_E0040) and the N-terminal alpha fragment of lacZ (which encodes β-galactosidase).  It is a reporter which can be measured via fluorescence from GFP or through a Miller assay of β-galactosidase.
 
This part is a fusion protein between GFP (BBa_E0040) and the N-terminal alpha fragment of lacZ (which encodes β-galactosidase).  It is a reporter which can be measured via fluorescence from GFP or through a Miller assay of β-galactosidase.
 +
 +
The GFP does not function well. See <bbpart>E0051</bbpart> for a better part.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:03, 2 March 2009

GFP.lacZalpha fusion

This part is a fusion protein between GFP (BBa_E0040) and the N-terminal alpha fragment of lacZ (which encodes β-galactosidase). It is a reporter which can be measured via fluorescence from GFP or through a Miller assay of β-galactosidase.

The GFP does not function well. See E0051 for a better part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644