Revision as of 06:35, 10 October 2021

RBS009

Description

Modulation of protein expression level

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

2021 SZPT-China

Biology

Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS009 (BBa_K3740008) using random primers and PCR, and the constructed plasmid J23100-RBS009-sfGFP-rrn B T1 (BBa_K3740060）. Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrn B T1 (BBa_K3740058)

Usage

Constructed J23100-RBS009-sfGFP-rrn B T1 (BBa_K3740060) was transformed into E. coli DH5α. we quantified the fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2

Characterization

The average fluorescence intensity of sfGFP induced by RBS009 (BBa_K3740008), was less than 3% of B0034 (BBa_B0034).

@@ Line 18: / Line 18: @@
 <p>Mutagenesis at specific position of RBS (<partinfo>BBa_B0034</partinfo>) was achieved RBS009 (<partinfo>BBa_K3740008</partinfo>) using random primers and PCR, and the constructed plasmid J23100-RBS009-sfGFP-rrn B T1 (<partinfo>BBa_K3740060</partinfo>）. Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrn B T1 (<partinfo>BBa_K3740058</partinfo>)</p>
 <br>
-<h3>Usage
+<h3>Usage</h3>
+Constructed J23100-RBS009-sfGFP-rrn B T1 (<partinfo>BBa_K3740060</partinfo>) was transformed into E. coli DH5α. we quantified the
+fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2
+<br>
+<h3>Characterization</h3>
+<p>The average fluorescence intensity of sfGFP induced by RBS009 (<partinfo>BBa_K3740008</partinfo>), was less than 3% of B0034 (<partinfo>BBa_B0034</partinfo>). </p>
+[[File:szpt0.png|300px|thumb|center]]