Difference between revisions of "Part:BBa K3889080"
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!Sequence
 
!Sequence
 
!Rel K<sub>D</sub>
 
!Rel K<sub>D</sub>
−
!K<sub>D</sub>
+
!K<sub>D</sub> (in M)
 
|-
 
|-
 
|P22 binding site A
 
|P22 binding site A
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|ATTGAAGATATCTTCAAT
 
|ATTGAAGATATCTTCAAT
 
|7.6
 
|7.6
−
|1.6 × 10<sup>-8</sup>
+
|1.216 × 10<sup>-8</sup>
 
|-
 
|-
 
|P22 binding site I
 
|P22 binding site I
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|P22 binding site K
 
|P22 binding site K
 
|<partinfo>BBa_K3889090</partinfo>
 
|<partinfo>BBa_K3889090</partinfo>
−
|ATTTACGATATGTTAAAT
+
|ATTTACGATATCGTAAAT
 
|30
 
|30
−
|1.6 × 10<sup>-7</sup>
+
|4.8 × 10<sup>-7</sup>
 
|-
 
|-
 
|P22 binding site L
 
|P22 binding site L

Revision as of 14:25, 9 October 2021


P22 binding site A

P22 c2 repressor DNA binding site.

Part Name Part number Sequence Rel KD KD (in M)
P22 binding site A BBa_K3889080 ATTTAAGATATCTTAAAT 1 1.6 × 10-8
P22 binding site B BBa_K3889081 AATTAAGATATCTTAATT 1.8 2.88 × 10-8
P22 binding site C BBa_K3889082 ATTTAAGAATTCTTAAAT 2 3.2 × 10-8
P22 binding site D BBa_K3889083 AGTTAAGATATCTTAACT 2.6 4.16 × 10-8
P22 binding site E BBa_K3889084 ATTAAAGATATCTTTAAT 3.8 6.08 × 10-8
P22 binding site F BBa_K3889085 ACTTAAGATATCTTAAGT 4.3 6.88 × 10-8
P22 binding site G BBa_K3889086 ATTCAAGATATCTTGAAT 5 8.0 × 10-8
P22 binding site H BBa_K3889087 ATTGAAGATATCTTCAAT 7.6 1.216 × 10-8
P22 binding site I BBa_K3889088 ATTTAAGAGCTCTTAAAT 10 1.6 × 10-7
P22 binding site J BBa_K3889089 ATTTAAGACGTCTTAAAT 10 1.6 × 10-7
P22 binding site K BBa_K3889090 ATTTACGATATCGTAAAT 30 4.8 × 10-7
P22 binding site L BBa_K3889091 ATTTAAAATATTTTAAAT 55 8.8 × 10-7


Fig.1:Kd values of P22 binding site


Usage and Biology

Different binding affinities of a repressor provides a variable system that can be used for different types of systems. P22 repressor (BBa_K3889020) binds to this sequence as a dimer. This inhibits the enzymes from transcription the genes on whose promoter this operator site is present. Hence this could be fused with any promoter in order to form a repressible system as done in BBa_K3889102

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Watkins, D., Hsiao, C., Woods, K. K., Koudelka, G. B., & Williams, L. D. (2008). P22 c2 Repressor−Operator Complex:  Mechanisms of Direct and Indirect Readout. Biochemistry, 47(8), 2325–2338. https://doi.org/10.1021/bi701826f