Difference between revisions of "Part:BBa K3889080"
Line 90: Line 90:
  
  
−
<!-- Add more about the biology of this part here
+
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
Different binding affinities of a repressor provides a variable system that can be used for different types of systems. P22 repressor (<partinfo>BBa_K3889020</partinfo>) binds to this sequence as a dimer. This inhibits the enzymes from transcription the genes on whose promoter this operator site is present. Hence this could be fused with any promoter in order to form a repressible system as done in <partinfo>BBa_K3889102</partinfo>
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889080 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889080 SequenceAndFeatures</partinfo>

Revision as of 11:06, 6 October 2021


P22 binding site A

P22 phage repressor protein DNA binding site.

Part Name Part number Sequence Rel KD KD
P22 binding site A BBa_K3889080 ATTTAAGATATCTTAAAT 1 1.6 × 10-8
P22 binding site B BBa_K3889081 AATTAAGATATCTTAATT 1.8 2.88 × 10-8
P22 binding site C BBa_K3889082 ATTTAAGAATTCTTAAAT 2 3.2 × 10-8
P22 binding site D BBa_K3889083 AGTTAAGATATCTTAACT 2.6 4.16 × 10-8
P22 binding site E BBa_K3889084 ATTAAAGATATCTTTAAT 3.8 6.08 × 10-8
P22 binding site F BBa_K3889085 ACTTAAGATATCTTAAGT 4.3 6.88 × 10-8
P22 binding site G BBa_K3889086 ATTCAAGATATCTTGAAT 5 8.0 × 10-8
P22 binding site H BBa_K3889087 ATTGAAGATATCTTCAAT 7.6 1.6 × 10-8
P22 binding site I BBa_K3889088 ATTTAAGAGCTCTTAAAT 10 1.6 × 10-7
P22 binding site J BBa_K3889089 ATTTAAGACGTCTTAAAT 10 1.6 × 10-7
P22 binding site K BBa_K3889090 ATTTACGATATGTTAAAT 30 1.6 × 10-7
P22 binding site L BBa_K3889091 ATTTAAAATATTTTAAAT 55 8.8 × 10-7


Fig.1:Kd values of P22 binding site


Usage and Biology

Different binding affinities of a repressor provides a variable system that can be used for different types of systems. P22 repressor (BBa_K3889020) binds to this sequence as a dimer. This inhibits the enzymes from transcription the genes on whose promoter this operator site is present. Hence this could be fused with any promoter in order to form a repressible system as done in BBa_K3889102

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Watkins, D., Hsiao, C., Woods, K. K., Koudelka, G. B., & Williams, L. D. (2008). P22 c2 Repressor−Operator Complex:  Mechanisms of Direct and Indirect Readout. Biochemistry, 47(8), 2325–2338. https://doi.org/10.1021/bi701826f