Difference between revisions of "Part:BBa K4047001"
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__NOTOC__
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<partinfo>BBa_K4047001 short</partinfo>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4047001 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4047001 parameters</partinfo>
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This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
 
This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.

Revision as of 18:00, 4 October 2021


primerT_tal-reverse


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.