Difference between revisions of "Part:BBa K4047002"
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__NOTOC__
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<partinfo>BBa_K4047002 short</partinfo>
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Reverse primer for FBPase
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4047002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4047002 parameters</partinfo>
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This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
 
This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.

Revision as of 17:56, 4 October 2021


primerF_fbp-foward

Reverse primer for FBPase

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.