Difference between revisions of "Part:BBa K4047002"

 
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This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
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__NOTOC__
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<partinfo>BBa_K4047002 short</partinfo>
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Forward primer for FBPase
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4047002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4047002 parameters</partinfo>
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Revision as of 17:55, 4 October 2021

This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.