Difference between revisions of "Part:BBa K4047000:Design"

(Design Notes)
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<partinfo>BBa_K4047000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4047000 SequenceAndFeatures</partinfo>
  
 
+
===Source===
 
Generated from a combination of factors.  
 
Generated from a combination of factors.  
 
Overlap sequence sourced from pAM2991.
 
Overlap sequence sourced from pAM2991.

Revision as of 17:54, 4 October 2021


primerT/TF_tal- forward


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Generated from a combination of factors. Overlap sequence sourced from pAM2991. https://www.addgene.org/40248/ Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for transaldolase, at the locus tag Synpcc7942_2297. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2367528&to=2368721&report=fasta&strand=2

References