Difference between revisions of "Part:BBa K3866001"
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The trc promoter is a hybrid of the lac and trp promoters which is a stronger promoter in comparison to the lac promoter. | The trc promoter is a hybrid of the lac and trp promoters which is a stronger promoter in comparison to the lac promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The trc promoter was created by insertion of 1 bp into the 16 bp sequence between the consensus -35 and -10 sequences of the tac promoter, to obtain the optimal consensus distance of 17 bp between the -35 and -10 signal. This promoter includes the <i>Lac Operator</i> sequence lacO, which can be bound by the Lac repressor lacI, as well as an RBS <bbpart>BBa_B0034</bbpart> | ||
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Revision as of 18:25, 25 September 2021
pTrc:RBS GB compatible with A1-B2
The trc promoter is a hybrid of the lac and trp promoters which is a stronger promoter in comparison to the lac promoter.
Usage and Biology
The trc promoter was created by insertion of 1 bp into the 16 bp sequence between the consensus -35 and -10 sequences of the tac promoter, to obtain the optimal consensus distance of 17 bp between the -35 and -10 signal. This promoter includes the Lac Operator sequence lacO, which can be bound by the Lac repressor lacI, as well as an RBS BBa_B0034
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]