Difference between revisions of "Part:BBa K3052002"

Latest revision as of 03:11, 28 October 2020

(S)-linalool synthase: LGF

The linalool synthase is used to convert geranyl pyrophosphate(GPP) to linalool, which has aroma of lavendar. This is a codon-optimized version based on E.coli.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 298
Illegal PstI site found at 817
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 298
Illegal PstI site found at 817
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 129
Illegal BglII site found at 1091
Illegal BglII site found at 1139
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 298
Illegal PstI site found at 817
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 298
Illegal PstI site found at 817
Illegal NgoMIV site found at 450
1000
COMPATIBLE WITH RFC[1000]

We have reached a cooperation with XJTU-China to test whether their LGF bacteria can work normally in 2019.
The LGF strain contains two plasmids, pGPP and pSB1K3-LGF. GPP can be synthesized by plasmid pGPP, while linalool can be synthesized by plasmid pSB1K3-LGF using GPP as substrate.
We qualitatively and quantitatively detected linalool in samples by gas chromatography. Test results are as follows:
(1) qualitative detection Chromatogram of linalool standard solution
<img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepubiao.jpg" />
The retention time of linalool in the standard solution was 8.455min, and the peak area was 788,307.
Chromatogram of linalool sample
<img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepuyangpin.jpg" />
The retention time of linalool in the sample was 8.431min, and the peak area was 632439. The sample contains a peak with a retention time close to that in the standard solution, which can be inferred as the product astragaloside.
(2) Quantitative detection
The quantitative measurement result of linalool is 377.66mg/ml.

@@ Line 12: / Line 12: @@
 <partinfo>BBa_K3052002 SequenceAndFeatures</partinfo>
 <p><br />We have reached a cooperation with XJTU-China to test whether their LGF bacteria can work normally in 2019.
-The LGF strain contains two plasmids, pGPP and pSB1K3-LGF. GPP can be synthesized by plasmid pGPP, while linalool can be synthesized by plasmid pSB1K3-LGF using GPP as substrate.
+<br />The LGF strain contains two plasmids, pGPP and pSB1K3-LGF. GPP can be synthesized by plasmid pGPP, while linalool can be synthesized by plasmid pSB1K3-LGF using GPP as substrate.
 <br />We qualitatively and quantitatively detected linalool in samples by gas chromatography.
@@ Line 19: / Line 19: @@
 Chromatogram of linalool standard solution
 <br /><img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepubiao.jpg" />
-The retention time of linalool in the standard solution was 8.455min, and the peak area was 788,307.
+<br />The retention time of linalool in the standard solution was 8.455min, and the peak area was 788,307.
 <br />Chromatogram of linalool sample
 <br /><img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepuyangpin.jpg" />
-The retention time of linalool in the sample was 8.431min, and the peak area was 632439. The sample contains a peak with a retention time close to that in the standard solution, which can be inferred as the product astragaloside.
+<br />The retention time of linalool in the sample was 8.431min, and the peak area was 632439. The sample contains a peak with a retention time close to that in the standard solution, which can be inferred as the product astragaloside.
 <br />(2) Quantitative detection
 <br />The quantitative measurement result of linalool is 377.66mg/ml.</p>