Difference between revisions of "Part:BBa K3657030"
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<p>N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (<a href="https://parts.igem.org/Part:BBa_K3657028">BBa_K3657028</a>).</p> | <p>N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (<a href="https://parts.igem.org/Part:BBa_K3657028">BBa_K3657028</a>).</p> | ||
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<p>This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: <a href="https://parts.igem.org/Part:BBa_K3657036">BBa_K3657036</a>. The part with the C-terminal of the Split-GFP planned for the experiment: <a href="https://parts.igem.org/Part:BBa_K3657031">BBa_K3657031</a>. You can find out more about the planned experiment here: <a href="https://2020.igem.org/Team:Heidelberg/RNA_Protein">iGEM Heidelberg 2020.</a></p> | <p>This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: <a href="https://parts.igem.org/Part:BBa_K3657036">BBa_K3657036</a>. The part with the C-terminal of the Split-GFP planned for the experiment: <a href="https://parts.igem.org/Part:BBa_K3657031">BBa_K3657031</a>. You can find out more about the planned experiment here: <a href="https://2020.igem.org/Team:Heidelberg/RNA_Protein">iGEM Heidelberg 2020.</a></p> | ||
<p> Due to the COVID19 pandemics we did not obtain empirical data about this part.</p> | <p> Due to the COVID19 pandemics we did not obtain empirical data about this part.</p> | ||
Latest revision as of 02:19, 28 October 2020
Antitermination protein N coupled with N terminal split GFP
This is a fusion protein containing Antitermination protein N (BBa_K3657014) and N-terminal Split-GFP (BBa_K3657027).
Usage and Biology
Antitermination protein N binds specifically the BoxB RNA (BBa_K3657015).
N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (BBa_K3657028).
This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the C-terminal of the Split-GFP planned for the experiment: BBa_K3657031. You can find out more about the planned experiment here: iGEM Heidelberg 2020.
Due to the COVID19 pandemics we did not obtain empirical data about this part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]