Difference between revisions of "Part:BBa K3657027"
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===Usage and Biology===
 
===Usage and Biology===
  
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Split-GFP was designed based on the Green Fluorescent Protein (GFP). It is made of two subunits. Both subunits do not emmit fluorescence by themselves. When the subunits are brought in close proximity of each other, they bind and form a fluorescent complex. If both subunits are co-expressed in one cell without an agent that brings them together, no fluorescence is observed.
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Split-GFP is mostly used to prove interaction between two proteins. To achieve that one should fuse proteins of interest with respectivly N- and C-Terminal subunits of the Split-GFP.
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We,<a href="https://2020.igem.org/Team:Heidelberg/RNA_Protein">iGEM Heidelberg 2020</a>, planned on using the Split-GFP to test the concept of RNA linker.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657027 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657027 SequenceAndFeatures</partinfo>

Revision as of 01:26, 28 October 2020


Split-GFP N-Terminal Subunit

This part is the sequence for the N-Terminal subunit of the Split-GFP. It is used with the C-Terminal subunit of the Split-GFP (BBa_K3657027).


Usage and Biology


Split-GFP was designed based on the Green Fluorescent Protein (GFP). It is made of two subunits. Both subunits do not emmit fluorescence by themselves. When the subunits are brought in close proximity of each other, they bind and form a fluorescent complex. If both subunits are co-expressed in one cell without an agent that brings them together, no fluorescence is observed.

Split-GFP is mostly used to prove interaction between two proteins. To achieve that one should fuse proteins of interest with respectivly N- and C-Terminal subunits of the Split-GFP. We,iGEM Heidelberg 2020, planned on using the Split-GFP to test the concept of RNA linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]