Difference between revisions of "Part:BBa K3505012"
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Constitutive Anderson <bbpart>BBa_J23115</bbpart>with RBS <bbpart>BBa_B0034</bbpart>
 
Constitutive Anderson <bbpart>BBa_J23115</bbpart>with RBS <bbpart>BBa_B0034</bbpart>
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AndersonJ23115 is a strong constitutive promoter  
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<p>AndersonJ23115 is a strong constitutive promoter that can be used to tune the expression level of constitutively expressed parts.
  
  

Revision as of 15:32, 27 October 2020


AndersonJ23115:RBS GB compatible with A1-B2


Constitutive Anderson BBa_J23115with RBS BBa_B0034

AndersonJ23115 is a strong constitutive promoter that can be used to tune the expression level of constitutively expressed parts.

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. This has position A1-B3.

Fig.2:The overhangs of this part in the Golden Braid Grammar
Fig.1:J23115 with RBS


Verification of cloning

Fig.1:((U=Uncut , C= Cut) Restriction digestion of AndersonJ23115 with : EcoRI (C1a & C2a),Expected bands : 2156 bp, XhoI (C1b & C2b),Expected bands : 1264 + 892 bp Positive result: The set of C1a and C1b (same sample)

Experimental Use and Experience

This part is used in BBa_K3505033 BBa_K3505035

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]