Difference between revisions of "Part:BBa Z0251:Experience"
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iGEM 2020 TEam Edinburgh has tested successfully tested this promoter. It's efficiency was not assessed in comparison to other T7 promoters. Nevertheless it was used in the <partinfo>BBa_K3380500</partinfo> construct to express the iSpinach RNA aptamer (<partinfo>BBa_K3380500</partinfo>) flanked by F30 tRNA scaffolds (<partinfo>BBa_K3380101</partinfo> and <partinfo>BBa_K3380102</partinfo>) some of the results can be seen below. | iGEM 2020 TEam Edinburgh has tested successfully tested this promoter. It's efficiency was not assessed in comparison to other T7 promoters. Nevertheless it was used in the <partinfo>BBa_K3380500</partinfo> construct to express the iSpinach RNA aptamer (<partinfo>BBa_K3380500</partinfo>) flanked by F30 tRNA scaffolds (<partinfo>BBa_K3380101</partinfo> and <partinfo>BBa_K3380102</partinfo>) some of the results can be seen below. | ||
| + | {| style="border-spacing: 2px; border: 1px solid darkgray;" | ||
| + | |- style="text-align: center;" | ||
| + | | style="border: 1px solid #777777;"| | ||
| + | [[File:BBa K3380500 1.jpeg|300px|5min]] | ||
| + | | style="border: 1px solid #777777;"| | ||
| + | [[File:Part BBa K3380500 2.jpeg|300px|10min]] | ||
| + | | style="border: 1px solid #777777;"| | ||
| + | [[File:Part BBa K3380500 3.jpeg|300px|15min]] | ||
| + | |- style="text-align: center;" | ||
| + | |5 minutes after fluorophore addition||10 minutes after fluorophore addition||15 min after fluorophore addition | ||
| + | |- style="text-align: center;" | ||
| + | | style="border: 1px solid #777777;"| | ||
| + | [[File:Part BBa K3380500 4.jpeg|300px|5min]] | ||
| + | | style="border: 1px solid #777777;"| | ||
| + | [[File:Part BBa K3380500 5.jpeg|300px|10min]] | ||
| + | | style="border: 1px solid #777777;"| | ||
| + | [[File:Part BBa K3380500 6.jpeg|300px|15min]] | ||
| + | |- style="text-align: center;" | ||
| + | |20 minutes after fluorophore addition||25 minutes after fluorophore addition||30 min after fluorophore addition | ||
| + | |} | ||
| + | '''Figure 2. Impact of DFHBI fluorophore addition to the construct BBa_K3380500 over time.''' Each time point has 3 PCR tubes: the first one containing the construct + DFHBI fluorophore, second once having the fluorophore and no construct and the 3rd one having no T7 RNA polymerase added. The fluorescence was imaged under a Safe Imager™ Blue-Light Transilluminator (Invitrogen) with an amber filter unit and a phone camera. The details regarding the assembling process can be found in the Experience page of this construct[https://parts.igem.org/Part:BBa_K3380500:Experience#In_vitro_Transcription_using_the_OTRDB_buffer]. | ||
|} | |} | ||
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Revision as of 14:44, 27 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_Z0251
User Reviews
UNIQ3b9ecce991cc50de-partinfo-00000000-QINU
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iGEM2020 Team Edinburgh |
iGEM 2020 TEam Edinburgh has tested successfully tested this promoter. It's efficiency was not assessed in comparison to other T7 promoters. Nevertheless it was used in the BBa_K3380500 construct to express the iSpinach RNA aptamer (BBa_K3380500) flanked by F30 tRNA scaffolds (BBa_K3380101 and BBa_K3380102) some of the results can be seen below.
Figure 2. Impact of DFHBI fluorophore addition to the construct BBa_K3380500 over time. Each time point has 3 PCR tubes: the first one containing the construct + DFHBI fluorophore, second once having the fluorophore and no construct and the 3rd one having no T7 RNA polymerase added. The fluorescence was imaged under a Safe Imager™ Blue-Light Transilluminator (Invitrogen) with an amber filter unit and a phone camera. The details regarding the assembling process can be found in the Experience page of this construct[1]. |
UNIQ3b9ecce991cc50de-partinfo-00000006-QINU