Difference between revisions of "Part:BBa K3505032"
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===Extra Engineering Use=== | ===Extra Engineering Use=== | ||
+ | [[File:T--Thessaly--fluo-te-le.png|700px|thumb|none|<i><b>Fig.3:</b>Validation that J23115-TetO <bbpart>BBa_K3505044</bbpart> and J23115-LacO fusion promoters are able to drive expression of ECFP.</i>]] | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K3505032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3505032 SequenceAndFeatures</partinfo> |
Revision as of 13:14, 27 October 2020
pAndersonJ23115:lacO:RBS-eCFP -terminator
eCFP BBa_K3505020uder control of a constitutive promoter (andersonJ23115 with a lac operator) BBa_K2924013.
Usage and Biology
This Trancriscription Unit (TU) is continuesly activated exressing the eCFP protein as a reporter. The Lac operator that is downsteam the anderson exists for the lac regulated inhibition.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in both a1R BBa_K3505008and a2 BBa_K3505009 and has overhangs compatible for Golden Braid cloning.
Verification of cloning
Experimental Use and Experinece
This part is used in BBa_K3505036
Extra Engineering Use
![](/wiki/images/2/2e/T--Thessaly--fluo-te-le.png)
Fig.3:Validation that J23115-TetO BBa_K3505044 and J23115-LacO fusion promoters are able to drive expression of ECFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]