Reporter

Part:BBa_K3505020

Designed by: Venetios Michelioudakis, Magdalini Koroxenidou, Foteini Papadaki   Group: iGEM20_Thessaly   (2020-10-20)


ECFP GB compatible with B3-B5

engineered cyan fluorescent protein derived from A. victoria GFP Level 0 eCFP

Fig.1:sfGFP



Usage and Biology

A reporter gene fluorescent protein

  • Excitation at 434 nm
  • Emission at 477 nm


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007and has overhangs compatible for GoldenBraid cloning. The CDS has position B3-B5.

Fig.2:The overhangs of this part in the GoldenBraid Grammar

Verification of cloning

Fig.3:: (U=Uncut C=Cut) Restriction Enzyme with SlaI, Expected bands in bp 1934 + 892

Experimental Use and Experience

This part is used in BBa_K3505028 BBa_K3505032 BBa_K3505036

Source

Synthesized by IDT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None