Difference between revisions of "Part:BBa K3599018"
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===Design & Experience===
 
===Design & Experience===
 
In iGEM2020 TPR_China Project, an aromatic sensor was constructed using Pa promoter, the eGFP ([[Part:BBa_K3599016|BBa_K3599016]]) and the sfGFP ([[Part:BBa_K3599017|BBa_K3599017]]) were both used as downstream reporters to form the sensor devices. To sense the aromatic compounds, PaaX under [[Part:BBa_K1031744|J23114]] constitutive promoter was used together.
 
In iGEM2020 TPR_China Project, an aromatic sensor was constructed using Pa promoter, the eGFP ([[Part:BBa_K3599016|BBa_K3599016]]) and the sfGFP ([[Part:BBa_K3599017|BBa_K3599017]]) were both used as downstream reporters to form the sensor devices. To sense the aromatic compounds, PaaX under [[Part:BBa_K1031744|J23114]] constitutive promoter was used together.
 +
 +
====The fluorescence of the sensor induced by small molecules====
 +
Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control.And here we can see that Xyls-sfGFP is 18 times of the Xyls-GFP( BBa_K1031911). And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before.<br>
 +
[[File:T--TPR China--Sensor1.png|600px|thumb|center|The fluorescence of the sensor induced by small molecules]]<br>
 +
[[File:T--TPR China--Sensor2.png|600px|thumb|center|The fluorescence of the NahR sensor induced by small molecules]]<br>
 +
 +
====The fluorescence of the sensor induced by PAN====
 +
And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best.
 +
[[File:T--TPR China--Sensor3.jpg|600px|thumb|center|The fluorescence of the sensor induced by PAN]]<br>

Revision as of 07:13, 27 October 2020


Pa


Overview

An induced promoter which could be regulated by transcription factor PaaX (BBa_K1031710).


Sequence And Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design & Experience

In iGEM2020 TPR_China Project, an aromatic sensor was constructed using Pa promoter, the eGFP (BBa_K3599016) and the sfGFP (BBa_K3599017) were both used as downstream reporters to form the sensor devices. To sense the aromatic compounds, PaaX under J23114 constitutive promoter was used together.

The fluorescence of the sensor induced by small molecules

Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control.And here we can see that Xyls-sfGFP is 18 times of the Xyls-GFP( BBa_K1031911). And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before.

The fluorescence of the sensor induced by small molecules

The fluorescence of the NahR sensor induced by small molecules

The fluorescence of the sensor induced by PAN

And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best.

The fluorescence of the sensor induced by PAN