Difference between revisions of "Part:BBa K3657025"
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| − | <p>The MS2 hairpin binds to the MS2 protein (<a href="https://parts.igem.org/Part:BBa_K3218006">BBa_K3218006</a>). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above. More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a></p> | + | <p>The MS2 hairpin binds to the MS2 protein (<a href="https://parts.igem.org/Part:BBa_K3218006">BBa_K3218006</a>). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above. More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a>.</p> |
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Revision as of 00:13, 27 October 2020
RNA Part of an RNA·DNA-DNA triple helix + MS2-Stemloop
This part consists of a sequence that forms an RNA·DNA-DNA triple helix (DNA part: BBa_K3657023) and a sequence that forms an MS2 hairpin (BBa_K3113006).
Usage and Biology
The MS2 hairpin binds to the MS2 protein (BBa_K3218006). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above. More information available here: iGEM Heidelberg 2020.
Figure 1: Secondary structure of BBa_K3657025
MS2 hairpin forms a stable structure. The triple helix forming sequence doesn't form any stable structure and does not interfere with the MS2 hairpin. The structure was generated by Geneious software.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]