Difference between revisions of "Part:BBa K3657025"

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	<b>Figure 1: Secondary structure of BBa_K3657025</b>		<b>Figure 1: Secondary structure of BBa_K3657025</b>

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Revision as of 00:10, 27 October 2020

RNA Part of an RNA·DNA-DNA triple helix + MS2-Stemloop

This part consists of a sequence that forms an RNA·DNA-DNA triple helix (DNA part: BBa_K3657023) and a sequence that forms an MS2 hairpin (BBa_K3113006).

Usage and Biology

The MS2 hairpin binds to the MS2 protein (BBa_K3218006). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above.
Figure 1: Secondary structure of BBa_K3657025 MS2 hairpin forms a stable structure. The triple helix forming sequence doesn't form any stable structure and does not interfere with the MS2 hairpin. The structure was generated by Geneious software.
Sequence and Features