Difference between revisions of "Part:BBa K3657025"

Revision as of 23:33, 26 October 2020 (view source) Liza ch (Talk | contribs) ← Older edit		Revision as of 23:48, 26 October 2020 (view source) Liza ch (Talk | contribs) Newer edit →
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		+	<h4>Usage and Biology</h4>
		+	The MS2 hairpin binds to the MS2 protein (<a href="https://parts.igem.org/Part:BBa_K3218006">BBa_K3218006</a>). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above.
	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>
	<partinfo>BBa_K3657025 SequenceAndFeatures</partinfo>		<partinfo>BBa_K3657025 SequenceAndFeatures</partinfo>

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Revision as of 23:48, 26 October 2020

RNA Part of an RNA·DNA-DNA triple helix + MS2-Stemloop

This part consists of a sequence that forms an RNA·DNA-DNA triple helix (DNA part: BBa_K3657023) and a sequence that forms an MS2 hairpin (BBa_K3113006).

Usage and Biology

The MS2 hairpin binds to the MS2 protein (BBa_K3218006). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above. Sequence and Features